{"gene":"PF3D7_0319600","run_date":"2026-09-07T10:55:51","timeline":{"discoveries":[{"year":2007,"finding":"PF3D7_0319600 (EF-1delta) was identified as a native subunit of the P. falciparum translation elongation factor complex, co-purifying with PfEF-1beta (tagged bait), native PfEF-1beta, PfEF-1alpha, and PfEF-1gamma, establishing its membership in this multi-subunit complex.","method":"Tandem affinity purification (TAP) of PfEF-1beta-PTP from transgenic P. falciparum extracts followed by mass spectrometric identification","journal":"Eukaryotic cell","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — TAP-MS under native conditions in P. falciparum, single lab, single method; no reciprocal pulldown or functional validation of EF-1delta specifically","pmids":["17307963"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"EF-1delta (PF3D7_0319600) is a subunit of the P. falciparum translation elongation factor complex, identified by its co-purification with PfEF-1alpha and PfEF-1beta via tandem affinity purification, with no further mechanistic characterization established in the available literature."},"narrative":{"mechanistic_narrative":"EF-1delta (PF3D7_0319600) is a native subunit of the Plasmodium falciparum translation elongation factor complex, identified by its co-purification with PfEF-1beta, PfEF-1alpha, and PfEF-1gamma during tandem affinity purification of a PfEF-1beta bait [PMID:17307963]. Beyond this evidence of complex membership, no function, substrate, localization, or phenotype specific to EF-1delta has been characterized in the available corpus.","teleology":[{"year":2007,"claim":"Whether PF3D7_0319600 physically associates with the parasite's core elongation machinery was unknown; tagging PfEF-1beta and purifying its native partners placed EF-1delta within the multi-subunit PfEF-1 complex, establishing it as a bona fide component of the elongation factor assembly.","evidence":"Tandem affinity purification of PfEF-1beta-PTP from transgenic P. falciparum extracts with mass spectrometric identification of co-purifying subunits","pmids":["17307963"],"confidence":"Medium","gaps":["No reciprocal pulldown using EF-1delta itself as bait to confirm the interaction directionally","No functional or biochemical role assigned to EF-1delta within the complex","Stoichiometry, binding interface, and direct partners of EF-1delta within the complex are unresolved"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"It remains unknown what specific molecular contribution EF-1delta makes to translation elongation in P. falciparum, whether it acts as a guanine-nucleotide exchange component, and what its localization and essentiality are.","evidence":"No further experimental characterization of PF3D7_0319600 in the available corpus","pmids":[],"confidence":"Medium","gaps":["No direct assay of EF-1delta catalytic or regulatory activity","No genetic knockout or knockdown to assess essentiality","No subcellular localization data for the protein"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["P. falciparum translation elongation factor 1 (PfEF-1) complex"],"partners":["PFEF-1BETA","PFEF-1ALPHA","PFEF-1GAMMA"],"other_free_text":["Identified solely as a co-purifying subunit of the PfEF-1 complex via TAP-MS with PfEF-1beta as bait; no EF-1delta-specific function established [#0]."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"17307963","id":"PMC_17307963","title":"Purification of components of the translation elongation factor complex of Plasmodium falciparum by tandem affinity purification.","date":"2007","source":"Eukaryotic cell","url":"https://pubmed.ncbi.nlm.nih.gov/17307963","citation_count":15,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3407,"output_tokens":393,"usd":0.008058,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4861,"output_tokens":1066,"usd":0.025478,"stage2_stop_reason":"end_turn"},"total_usd":0.033536,"stage1_batch_id":"msgbatch_01DbiymDmubkPpYvdbAu5a7V","stage2_batch_id":"msgbatch_012TbrWvMRH129T7PQ4SmvrJ","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2007,\n      \"finding\": \"PF3D7_0319600 (EF-1delta) was identified as a native subunit of the P. falciparum translation elongation factor complex, co-purifying with PfEF-1beta (tagged bait), native PfEF-1beta, PfEF-1alpha, and PfEF-1gamma, establishing its membership in this multi-subunit complex.\",\n      \"method\": \"Tandem affinity purification (TAP) of PfEF-1beta-PTP from transgenic P. falciparum extracts followed by mass spectrometric identification\",\n      \"journal\": \"Eukaryotic cell\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — TAP-MS under native conditions in P. falciparum, single lab, single method; no reciprocal pulldown or functional validation of EF-1delta specifically\",\n      \"pmids\": [\"17307963\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"EF-1delta (PF3D7_0319600) is a subunit of the P. falciparum translation elongation factor complex, identified by its co-purification with PfEF-1alpha and PfEF-1beta via tandem affinity purification, with no further mechanistic characterization established in the available literature.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"EF-1delta (PF3D7_0319600) is a native subunit of the Plasmodium falciparum translation elongation factor complex, identified by its co-purification with PfEF-1beta, PfEF-1alpha, and PfEF-1gamma during tandem affinity purification of a PfEF-1beta bait [#0]. Beyond this evidence of complex membership, no function, substrate, localization, or phenotype specific to EF-1delta has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2007,\n      \"claim\": \"Whether PF3D7_0319600 physically associates with the parasite's core elongation machinery was unknown; tagging PfEF-1beta and purifying its native partners placed EF-1delta within the multi-subunit PfEF-1 complex, establishing it as a bona fide component of the elongation factor assembly.\",\n      \"evidence\": \"Tandem affinity purification of PfEF-1beta-PTP from transgenic P. falciparum extracts with mass spectrometric identification of co-purifying subunits\",\n      \"pmids\": [\"17307963\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No reciprocal pulldown using EF-1delta itself as bait to confirm the interaction directionally\",\n        \"No functional or biochemical role assigned to EF-1delta within the complex\",\n        \"Stoichiometry, binding interface, and direct partners of EF-1delta within the complex are unresolved\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"It remains unknown what specific molecular contribution EF-1delta makes to translation elongation in P. falciparum, whether it acts as a guanine-nucleotide exchange component, and what its localization and essentiality are.\",\n      \"evidence\": \"No further experimental characterization of PF3D7_0319600 in the available corpus\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No direct assay of EF-1delta catalytic or regulatory activity\",\n        \"No genetic knockout or knockdown to assess essentiality\",\n        \"No subcellular localization data for the protein\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [],\n    \"pathway\": [\n      {\"term_id\": \"GO:0006414\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"complexes\": [\"P. falciparum translation elongation factor 1 (PfEF-1) complex\"],\n    \"partners\": [\"PfEF-1beta\", \"PfEF-1alpha\", \"PfEF-1gamma\"],\n    \"other_free_text\": [\n      \"Identified solely as a co-purifying subunit of the PfEF-1 complex via TAP-MS with PfEF-1beta as bait; no EF-1delta-specific function established [#0].\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0319600","name":null,"product":"elongation factor 1-delta, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1218900","name":null,"product":"elongation factor 1-delta, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0821600","name":null,"product":"elongation factor 1-delta, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"EF-1delta","evaluation":null}