{"gene":"PF3D7_0318200","run_date":"2026-09-07T10:55:51","timeline":{"discoveries":[{"year":1989,"finding":"The largest subunit of RNA polymerase II (RPB1) in P. falciparum is encoded by a single-copy gene located on chromosome 3, is expressed in asexual erythrocytic stages as a 9 kb mRNA, and encodes an unusually large protein of 2452 amino acids — the largest eukaryotic RPII largest subunit known — containing enlarged variable regions that define five conserved domains, a conserved C-terminal heptapeptide repeat domain, a diverged heptapeptide repeat domain on its 5' side, and a long C-terminal extension.","method":"Gene isolation, Southern blotting (single-copy, chromosome 3 localization), Northern blotting (9 kb mRNA in asexual stages), sequence analysis of the RPII gene","journal":"Nucleic acids research","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — direct gene isolation and sequencing with Northern and Southern blot validation in a single study; no functional mutagenesis","pmids":["2690004"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2014,"finding":"The C-terminal domain (CTD) of RPB1 (RNA polymerase II largest subunit) directly binds PfSET2 in its unphosphorylated form; this interaction recruits PfSET2 to var gene loci, where it deposits H3K36me3 at both active and silent loci. Over-expression of a dominant-negative PfSET2 designed to disrupt CTD binding caused rapid var gene expression switching, establishing that RPB1's CTD-mediated recruitment of PfSET2 contributes to antigenic variation.","method":"Co-immunoprecipitation (RPB1 CTD–PfSET2 interaction), dominant-negative over-expression, chromatin immunoprecipitation (H3K36me3 enrichment at var loci), var switching assay","journal":"PLoS pathogens","confidence":"High","confidence_rationale":"Tier 2 / Strong — reciprocal interaction shown by Co-IP, functional consequence confirmed by dominant-negative perturbation and ChIP, multiple orthogonal methods in one study","pmids":["24391504"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2005,"finding":"RNA polymerase II (containing RPB1) synthesizes antisense RNA in P. falciparum nuclei; this antisense transcription is sensitive to α-amanitin (a potent Pol II inhibitor) and occurs concurrently with sense transcription at multiple genomic loci.","method":"Nuclear run-on assay with single-stranded DNA probes; α-amanitin inhibition; synchronized parasite nuclei preparation","journal":"RNA (New York, N.Y.)","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — direct nuclear run-on with α-amanitin pharmacological validation, single lab, two orthogonal approaches (probe specificity + inhibitor)","pmids":["15703443"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2017,"finding":"RNA polymerase II (containing RPB1) is engaged at promoter regions prior to the major transcriptional burst during the trophozoite stage of the intraerythrocytic developmental cycle, consistent with a Pol II pausing mechanism that regulates the timing of gene expression genome-wide.","method":"Genome-wide nascent RNA sequencing (GRO-seq/equivalent) in synchronized P. falciparum parasites","journal":"Nucleic acids research","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — genome-wide nascent RNA profiling provides direct evidence of Pol II promoter engagement; single lab, single method","pmids":["28531310"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"P. falciparum RPB1 encodes an unusually large (2452 aa) single-copy RNA polymerase II largest subunit expressed in asexual blood stages; its unphosphorylated CTD directly recruits the histone methyltransferase PfSET2 to var gene loci to deposit H3K36me3 and regulate antigenic variation, while the polymerase itself synthesizes both sense and antisense RNAs in the nucleus and pauses at promoters genome-wide to gate the trophozoite-stage transcriptional burst."},"narrative":{"mechanistic_narrative":"PF3D7_0318200 (RPB1) encodes the largest subunit of RNA polymerase II in Plasmodium falciparum, a single-copy, chromosome 3 gene expressed as a 9 kb mRNA in asexual erythrocytic stages that produces an unusually large 2452-amino-acid protein bearing enlarged variable regions and both a conserved and a diverged C-terminal heptapeptide repeat domain [PMID:2690004]. Beyond its catalytic role in transcription, the unphosphorylated C-terminal domain (CTD) of RPB1 directly binds the histone methyltransferase PfSET2 and recruits it to var gene loci, where H3K36me3 is deposited at both active and silent loci; disrupting this CTD-mediated recruitment triggers rapid var gene switching, linking RPB1 to the control of antigenic variation [PMID:24391504]. The polymerase is transcriptionally active in parasite nuclei, generating antisense as well as sense RNA in an α-amanitin-sensitive manner [PMID:15703443], and it is engaged at promoters prior to the trophozoite-stage transcriptional burst, consistent with a Pol II pausing mechanism that gates the timing of genome-wide gene expression [PMID:28531310]. Beyond these findings, no further mechanistic detail specific to RPB1 has been characterized in the available corpus.","teleology":[{"year":1989,"claim":"Establishing whether P. falciparum possesses a canonical Pol II largest subunit answered a basic question about the parasite's transcription machinery and revealed an atypically enlarged protein.","evidence":"Gene isolation with Southern and Northern blotting plus sequence analysis of the RPII gene in P. falciparum","pmids":["2690004"],"confidence":"Medium","gaps":["No functional or catalytic characterization of the encoded protein","Role of the enlarged variable regions and long C-terminal extension unknown","CTD heptapeptide repeat function not tested"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2005,"claim":"Whether Pol II drives the pervasive antisense transcription observed in the parasite was resolved by showing RPB1-containing polymerase generates antisense RNA sensitive to a Pol II inhibitor.","evidence":"Nuclear run-on assay with strand-specific probes and α-amanitin inhibition in synchronized P. falciparum nuclei","pmids":["15703443"],"confidence":"Medium","gaps":["Genomic scope and regulatory function of the antisense transcripts not defined","Single lab, single-method (pharmacological) attribution to Pol II"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2014,"claim":"How chromatin marks are targeted to var loci was clarified by showing the unphosphorylated RPB1 CTD recruits PfSET2 to deposit H3K36me3, connecting the polymerase to antigenic variation.","evidence":"Co-IP of RPB1 CTD with PfSET2, dominant-negative over-expression disrupting CTD binding, ChIP for H3K36me3, and var switching assays","pmids":["24391504"],"confidence":"High","gaps":["Structural basis of the CTD–PfSET2 contact not resolved","How CTD phosphorylation state is regulated to toggle recruitment unknown","Whether recruitment differs mechanistically between active and silent var loci not defined"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2017,"claim":"The mechanism timing the trophozoite transcriptional burst was addressed by showing Pol II is engaged at promoters before the burst, consistent with promoter pausing.","evidence":"Genome-wide nascent RNA sequencing in synchronized P. falciparum parasites","pmids":["28531310"],"confidence":"Medium","gaps":["Pause-release factors and triggers not identified","Direct role of RPB1 features in pausing not tested","Single lab, single method"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"How RPB1's atypical enlarged domains and CTD phosphorylation cycle are mechanistically coupled to promoter pausing and PfSET2-mediated var regulation remains unresolved.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No structural model of P. falciparum RPB1 or its CTD","No characterization of CTD kinases/phosphatases governing PfSET2 handoff","Direct link between promoter pausing and downstream regulatory outcomes untested"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["RNA polymerase II (largest subunit)"],"partners":["PFSET2 (HISTONE METHYLTRANSFERASE; BINDS UNPHOSPHORYLATED RPB1 CTD)"],"other_free_text":["Single-copy gene on chromosome 3 expressed as a 9 kb mRNA in asexual erythrocytic stages [#0]","Encodes an unusually large 2452-aa protein with conserved and diverged CTD heptapeptide repeats [#0]","Antisense RNA synthesis is α-amanitin-sensitive, confirming Pol II dependence [#2]","Promoter-engaged Pol II precedes the trophozoite-stage transcriptional burst, consistent with pausing [#3]","Dominant-negative disruption of CTD–PfSET2 binding causes rapid var gene switching [#1]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"10693754","id":"PMC_10693754","title":"A simple RNA analysis method shows var and rif multigene family expression patterns in Plasmodium falciparum.","date":"2000","source":"Molecular and biochemical parasitology","url":"https://pubmed.ncbi.nlm.nih.gov/10693754","citation_count":234,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["RNA"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"2690004","id":"PMC_2690004","title":"An enlarged largest subunit of Plasmodium falciparum RNA polymerase II defines conserved and variable RNA polymerase domains.","date":"1989","source":"Nucleic acids research","url":"https://pubmed.ncbi.nlm.nih.gov/2690004","citation_count":71,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["RNA"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"12032239","id":"PMC_12032239","title":"Analyses of RNA Polymerase II genes from free-living protists: phylogeny, long branch attraction, and the eukaryotic big bang.","date":"2002","source":"Molecular biology and evolution","url":"https://pubmed.ncbi.nlm.nih.gov/12032239","citation_count":62,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["RPB1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"24391504","id":"PMC_24391504","title":"Recruitment of PfSET2 by RNA polymerase II to variant antigen encoding loci contributes to antigenic variation in P. falciparum.","date":"2014","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/24391504","citation_count":59,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"4971014","id":"PMC_4971014","title":"DNA, RNA , and protein synthesis in erythrocytic forms of Plasmodium knowlesi.","date":"1968","source":"The American journal of tropical medicine and hygiene","url":"https://pubmed.ncbi.nlm.nih.gov/4971014","citation_count":56,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["DNA","RNA"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"15703443","id":"PMC_15703443","title":"RNA polymerase II synthesizes antisense RNA in Plasmodium falciparum.","date":"2005","source":"RNA (New York, N.Y.)","url":"https://pubmed.ncbi.nlm.nih.gov/15703443","citation_count":53,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["RNA"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"28531310","id":"PMC_28531310","title":"Nascent RNA sequencing reveals mechanisms of gene regulation in the human malaria parasite Plasmodium falciparum.","date":"2017","source":"Nucleic acids research","url":"https://pubmed.ncbi.nlm.nih.gov/28531310","citation_count":52,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["RNA"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"7976752","id":"PMC_7976752","title":"The ribosomal RNA genes of Plasmodium.","date":"1994","source":"Advances in parasitology","url":"https://pubmed.ncbi.nlm.nih.gov/7976752","citation_count":46,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["RNA"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"40626563","id":"PMC_40626563","title":"The ribosomal RNA transcription landscapes of Plasmodium falciparum and related apicomplexan parasites.","date":"2025","source":"Nucleic acids research","url":"https://pubmed.ncbi.nlm.nih.gov/40626563","citation_count":2,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["RNA"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":5847,"output_tokens":1275,"usd":0.018333,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6237,"output_tokens":2518,"usd":0.047067,"stage2_stop_reason":"end_turn"},"total_usd":0.0654,"stage1_batch_id":"msgbatch_01DbiymDmubkPpYvdbAu5a7V","stage2_batch_id":"msgbatch_012TbrWvMRH129T7PQ4SmvrJ","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 1989,\n      \"finding\": \"The largest subunit of RNA polymerase II (RPB1) in P. falciparum is encoded by a single-copy gene located on chromosome 3, is expressed in asexual erythrocytic stages as a 9 kb mRNA, and encodes an unusually large protein of 2452 amino acids — the largest eukaryotic RPII largest subunit known — containing enlarged variable regions that define five conserved domains, a conserved C-terminal heptapeptide repeat domain, a diverged heptapeptide repeat domain on its 5' side, and a long C-terminal extension.\",\n      \"method\": \"Gene isolation, Southern blotting (single-copy, chromosome 3 localization), Northern blotting (9 kb mRNA in asexual stages), sequence analysis of the RPII gene\",\n      \"journal\": \"Nucleic acids research\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — direct gene isolation and sequencing with Northern and Southern blot validation in a single study; no functional mutagenesis\",\n      \"pmids\": [\"2690004\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2014,\n      \"finding\": \"The C-terminal domain (CTD) of RPB1 (RNA polymerase II largest subunit) directly binds PfSET2 in its unphosphorylated form; this interaction recruits PfSET2 to var gene loci, where it deposits H3K36me3 at both active and silent loci. Over-expression of a dominant-negative PfSET2 designed to disrupt CTD binding caused rapid var gene expression switching, establishing that RPB1's CTD-mediated recruitment of PfSET2 contributes to antigenic variation.\",\n      \"method\": \"Co-immunoprecipitation (RPB1 CTD–PfSET2 interaction), dominant-negative over-expression, chromatin immunoprecipitation (H3K36me3 enrichment at var loci), var switching assay\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Strong — reciprocal interaction shown by Co-IP, functional consequence confirmed by dominant-negative perturbation and ChIP, multiple orthogonal methods in one study\",\n      \"pmids\": [\"24391504\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2005,\n      \"finding\": \"RNA polymerase II (containing RPB1) synthesizes antisense RNA in P. falciparum nuclei; this antisense transcription is sensitive to α-amanitin (a potent Pol II inhibitor) and occurs concurrently with sense transcription at multiple genomic loci.\",\n      \"method\": \"Nuclear run-on assay with single-stranded DNA probes; α-amanitin inhibition; synchronized parasite nuclei preparation\",\n      \"journal\": \"RNA (New York, N.Y.)\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — direct nuclear run-on with α-amanitin pharmacological validation, single lab, two orthogonal approaches (probe specificity + inhibitor)\",\n      \"pmids\": [\"15703443\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2017,\n      \"finding\": \"RNA polymerase II (containing RPB1) is engaged at promoter regions prior to the major transcriptional burst during the trophozoite stage of the intraerythrocytic developmental cycle, consistent with a Pol II pausing mechanism that regulates the timing of gene expression genome-wide.\",\n      \"method\": \"Genome-wide nascent RNA sequencing (GRO-seq/equivalent) in synchronized P. falciparum parasites\",\n      \"journal\": \"Nucleic acids research\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — genome-wide nascent RNA profiling provides direct evidence of Pol II promoter engagement; single lab, single method\",\n      \"pmids\": [\"28531310\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"P. falciparum RPB1 encodes an unusually large (2452 aa) single-copy RNA polymerase II largest subunit expressed in asexual blood stages; its unphosphorylated CTD directly recruits the histone methyltransferase PfSET2 to var gene loci to deposit H3K36me3 and regulate antigenic variation, while the polymerase itself synthesizes both sense and antisense RNAs in the nucleus and pauses at promoters genome-wide to gate the trophozoite-stage transcriptional burst.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_0318200 (RPB1) encodes the largest subunit of RNA polymerase II in Plasmodium falciparum, a single-copy, chromosome 3 gene expressed as a 9 kb mRNA in asexual erythrocytic stages that produces an unusually large 2452-amino-acid protein bearing enlarged variable regions and both a conserved and a diverged C-terminal heptapeptide repeat domain [#0]. Beyond its catalytic role in transcription, the unphosphorylated C-terminal domain (CTD) of RPB1 directly binds the histone methyltransferase PfSET2 and recruits it to var gene loci, where H3K36me3 is deposited at both active and silent loci; disrupting this CTD-mediated recruitment triggers rapid var gene switching, linking RPB1 to the control of antigenic variation [#1]. The polymerase is transcriptionally active in parasite nuclei, generating antisense as well as sense RNA in an \\u03b1-amanitin-sensitive manner [#2], and it is engaged at promoters prior to the trophozoite-stage transcriptional burst, consistent with a Pol II pausing mechanism that gates the timing of genome-wide gene expression [#3]. Beyond these findings, no further mechanistic detail specific to RPB1 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 1989,\n      \"claim\": \"Establishing whether P. falciparum possesses a canonical Pol II largest subunit answered a basic question about the parasite's transcription machinery and revealed an atypically enlarged protein.\",\n      \"evidence\": \"Gene isolation with Southern and Northern blotting plus sequence analysis of the RPII gene in P. falciparum\",\n      \"pmids\": [\n        \"2690004\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No functional or catalytic characterization of the encoded protein\",\n        \"Role of the enlarged variable regions and long C-terminal extension unknown\",\n        \"CTD heptapeptide repeat function not tested\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": 2005,\n      \"claim\": \"Whether Pol II drives the pervasive antisense transcription observed in the parasite was resolved by showing RPB1-containing polymerase generates antisense RNA sensitive to a Pol II inhibitor.\",\n      \"evidence\": \"Nuclear run-on assay with strand-specific probes and \\u03b1-amanitin inhibition in synchronized P. falciparum nuclei\",\n      \"pmids\": [\n        \"15703443\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Genomic scope and regulatory function of the antisense transcripts not defined\",\n        \"Single lab, single-method (pharmacological) attribution to Pol II\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": 2014,\n      \"claim\": \"How chromatin marks are targeted to var loci was clarified by showing the unphosphorylated RPB1 CTD recruits PfSET2 to deposit H3K36me3, connecting the polymerase to antigenic variation.\",\n      \"evidence\": \"Co-IP of RPB1 CTD with PfSET2, dominant-negative over-expression disrupting CTD binding, ChIP for H3K36me3, and var switching assays\",\n      \"pmids\": [\n        \"24391504\"\n      ],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Structural basis of the CTD\\u2013PfSET2 contact not resolved\",\n        \"How CTD phosphorylation state is regulated to toggle recruitment unknown\",\n        \"Whether recruitment differs mechanistically between active and silent var loci not defined\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": 2017,\n      \"claim\": \"The mechanism timing the trophozoite transcriptional burst was addressed by showing Pol II is engaged at promoters before the burst, consistent with promoter pausing.\",\n      \"evidence\": \"Genome-wide nascent RNA sequencing in synchronized P. falciparum parasites\",\n      \"pmids\": [\n        \"28531310\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Pause-release factors and triggers not identified\",\n        \"Direct role of RPB1 features in pausing not tested\",\n        \"Single lab, single method\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"How RPB1's atypical enlarged domains and CTD phosphorylation cycle are mechanistically coupled to promoter pausing and PfSET2-mediated var regulation remains unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No structural model of P. falciparum RPB1 or its CTD\",\n        \"No characterization of CTD kinases/phosphatases governing PfSET2 handoff\",\n        \"Direct link between promoter pausing and downstream regulatory outcomes untested\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\n        \"term_id\": \"GO:0003899\",\n        \"supporting_discovery_ids\": [\n          0,\n          2,\n          3\n        ]\n      },\n      {\n        \"term_id\": \"GO:0003682\",\n        \"supporting_discovery_ids\": [\n          1\n        ]\n      }\n    ],\n    \"localization\": [\n      {\n        \"term_id\": \"GO:0005634\",\n        \"supporting_discovery_ids\": [\n          2\n        ]\n      }\n    ],\n    \"pathway\": [\n      {\n        \"term_id\": \"GO:0006366\",\n        \"supporting_discovery_ids\": [\n          2,\n          3\n        ]\n      },\n      {\n        \"term_id\": \"GO:0016571\",\n        \"supporting_discovery_ids\": [\n          1\n        ]\n      }\n    ],\n    \"complexes\": [\n      \"RNA polymerase II (largest subunit)\"\n    ],\n    \"partners\": [\n      \"PfSET2 (histone methyltransferase; binds unphosphorylated RPB1 CTD)\"\n    ],\n    \"other_free_text\": [\n      \"Single-copy gene on chromosome 3 expressed as a 9 kb mRNA in asexual erythrocytic stages [#0]\",\n      \"Encodes an unusually large 2452-aa protein with conserved and diverged CTD heptapeptide repeats [#0]\",\n      \"Antisense RNA synthesis is \\u03b1-amanitin-sensitive, confirming Pol II dependence [#2]\",\n      \"Promoter-engaged Pol II precedes the trophozoite-stage transcriptional burst, consistent with pausing [#3]\",\n      \"Dominant-negative disruption of CTD\\u2013PfSET2 binding causes rapid var gene switching [#1]\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0318200","name":null,"product":"DNA-directed RNA polymerase II subunit RPB1","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0807000","name":null,"product":"DNA-directed RNA polymerase II subunit RPB1, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0823500","name":null,"product":"DNA-directed RNA polymerase II subunit RPB1, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"RPB1","evaluation":null}