{"gene":"PF3D7_0306500","run_date":"2026-09-08T16:26:55","timeline":{"discoveries":[{"year":2021,"finding":"COX6C (PF3D7_0306500) was identified as a component of respiratory chain complex IV (cytochrome c oxidase) in Plasmodium falciparum through complexome profiling, establishing its membership in the mitochondrial respiratory chain complex.","method":"Complexome profiling (co-migration/mass spectrometry-based protein complex mapping) across asexual blood stages and gametocyte stages","journal":"Nature communications","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — complexome profiling is a systematic co-migration MS approach; single lab but maps complex membership directly; no reconstitution or mutagenesis","pmids":["34155201"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"COX6C (PF3D7_0306500) is a subunit of the divergently composed mitochondrial respiratory chain complex IV (cytochrome c oxidase) in Plasmodium falciparum, with the complex and its components being substantially more prevalent in gametocytes than in asexual blood stages."},"narrative":{"mechanistic_narrative":"COX6C (PF3D7_0306500) is a component of the mitochondrial respiratory chain complex IV (cytochrome c oxidase) in Plasmodium falciparum, identified through complexome profiling that mapped its co-migration with the assembled complex across asexual blood stages and gametocytes [PMID:34155201]. The available corpus establishes only this membership in complex IV; beyond its assignment to the cytochrome c oxidase complex [PMID:34155201], no further mechanistic detail — molecular activity, assembly role, or stage-specific function specific to PF3D7_0306500 — has been characterized.","teleology":[{"year":2021,"claim":"Whether PF3D7_0306500 participates in the P. falciparum respiratory chain was unknown; complexome profiling answered this by placing it within assembled complex IV, establishing its membership in the mitochondrial cytochrome c oxidase.","evidence":"Complexome profiling (co-migration/mass spectrometry-based complex mapping) across asexual blood and gametocyte stages in P. falciparum","pmids":["34155201"],"confidence":"Medium","gaps":["No mutagenesis or reconstitution to define the functional contribution of PF3D7_0306500 to complex IV activity","Molecular activity and structural placement of the subunit within the complex not resolved","Stage-specific dependence and essentiality of this individual subunit not tested"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"The specific function, essentiality, and mechanistic contribution of PF3D7_0306500 to cytochrome c oxidase assembly or activity remain undefined in the available corpus.","evidence":"No further direct experimental evidence on this gene in the timeline","pmids":[],"confidence":"Low","gaps":["No genetic knockout or knockdown phenotype for PF3D7_0306500","No biochemical assay isolating its role in electron transfer or complex assembly","No structural model localizing the subunit within complex IV"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Mitochondrial respiratory chain complex IV (cytochrome c oxidase)"],"partners":[],"other_free_text":["Identified as a subunit of P. falciparum complex IV by complexome profiling [#0]; the complex and its components are more prevalent in gametocytes than in asexual blood stages [#0]."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"34155201","id":"PMC_34155201","title":"Composition and stage dynamics of mitochondrial complexes in Plasmodium falciparum.","date":"2021","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/34155201","citation_count":92,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"39794322","id":"","title":"Girolline is a sequence context-selective modulator of eIF5A activity.","date":"2025","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/39794322","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["COX6C"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3996,"output_tokens":336,"usd":0.008514,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4824,"output_tokens":1002,"usd":0.024585,"stage2_stop_reason":"end_turn"},"total_usd":0.033099,"stage1_batch_id":"msgbatch_01NE1MTa8x1hW7Lo4tAaNzY1","stage2_batch_id":"msgbatch_0141vaQdeFvbo2q39AinvXM9","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2021,\n      \"finding\": \"COX6C (PF3D7_0306500) was identified as a component of respiratory chain complex IV (cytochrome c oxidase) in Plasmodium falciparum through complexome profiling, establishing its membership in the mitochondrial respiratory chain complex.\",\n      \"method\": \"Complexome profiling (co-migration/mass spectrometry-based protein complex mapping) across asexual blood stages and gametocyte stages\",\n      \"journal\": \"Nature communications\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — complexome profiling is a systematic co-migration MS approach; single lab but maps complex membership directly; no reconstitution or mutagenesis\",\n      \"pmids\": [\"34155201\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"COX6C (PF3D7_0306500) is a subunit of the divergently composed mitochondrial respiratory chain complex IV (cytochrome c oxidase) in Plasmodium falciparum, with the complex and its components being substantially more prevalent in gametocytes than in asexual blood stages.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"COX6C (PF3D7_0306500) is a component of the mitochondrial respiratory chain complex IV (cytochrome c oxidase) in Plasmodium falciparum, identified through complexome profiling that mapped its co-migration with the assembled complex across asexual blood stages and gametocytes [#0]. The available corpus establishes only this membership in complex IV; beyond its assignment to the cytochrome c oxidase complex [#0], no further mechanistic detail — molecular activity, assembly role, or stage-specific function specific to PF3D7_0306500 — has been characterized.\",\n  \"teleology\": [\n    {\n      \"year\": 2021,\n      \"claim\": \"Whether PF3D7_0306500 participates in the P. falciparum respiratory chain was unknown; complexome profiling answered this by placing it within assembled complex IV, establishing its membership in the mitochondrial cytochrome c oxidase.\",\n      \"evidence\": \"Complexome profiling (co-migration/mass spectrometry-based complex mapping) across asexual blood and gametocyte stages in P. falciparum\",\n      \"pmids\": [\"34155201\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No mutagenesis or reconstitution to define the functional contribution of PF3D7_0306500 to complex IV activity\",\n        \"Molecular activity and structural placement of the subunit within the complex not resolved\",\n        \"Stage-specific dependence and essentiality of this individual subunit not tested\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The specific function, essentiality, and mechanistic contribution of PF3D7_0306500 to cytochrome c oxidase assembly or activity remain undefined in the available corpus.\",\n      \"evidence\": \"No further direct experimental evidence on this gene in the timeline\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No genetic knockout or knockdown phenotype for PF3D7_0306500\",\n        \"No biochemical assay isolating its role in electron transfer or complex assembly\",\n        \"No structural model localizing the subunit within complex IV\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [],\n    \"pathway\": [],\n    \"complexes\": [\"Mitochondrial respiratory chain complex IV (cytochrome c oxidase)\"],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Identified as a subunit of P. falciparum complex IV by complexome profiling [#0]; the complex and its components are more prevalent in gametocytes than in asexual blood stages [#0].\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0306500","name":null,"product":"cytochrome c oxidase subunit 6c, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0404900","name":null,"product":"cytochrome c oxidase subunit 6c, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0836200","name":null,"product":"cytochrome c oxidase subunit 6c, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"COX6C","evaluation":null}