{"gene":"PF3D7_0215800","run_date":"2026-09-08T16:26:55","timeline":{"discoveries":[{"year":2008,"finding":"P. falciparum ORC5 (PfORC5) forms distinct nuclear foci that colocalize with the replication foci marker PCNA (PfPCNA) during early-to-mid trophozoite stage (actively replicating parasites), and co-immunoprecipitates with PCNA at this stage; the two proteins separate at the non-replicating late schizont stage.","method":"Immunofluorescence colocalization and co-immunoprecipitation (Co-IP) in P. falciparum-infected erythrocytes","journal":"Molecular microbiology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — reciprocal Co-IP and colocalization, single lab, two orthogonal methods","pmids":["18554328"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2008,"finding":"PfORC5 colocalizes with PfORC1 and PfPCNA at the onset of DNA replication (early trophozoite), establishing ORC5 as part of the replication foci machinery in the replication factory model.","method":"Immunofluorescence colocalization in P. falciparum-infected erythrocytes","journal":"Molecular microbiology","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — colocalization by immunofluorescence, single lab, consistent with Co-IP data from same study","pmids":["18554328"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2008,"finding":"Treatment of P. falciparum with the DNA replication inhibitor hydroxyurea disrupts ORC5/PCNA foci formation and inhibits parasitic growth, linking ORC5 nuclear foci to active DNA replication.","method":"Hydroxyurea drug treatment with immunofluorescence and growth assay","journal":"Molecular microbiology","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — chemical perturbation with cellular readout, single lab, two orthogonal readouts (foci and growth)","pmids":["18554328"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2008,"finding":"PfORC5 was identified as a functional homologue of S. cerevisiae ORC5 by bioinformatics and yeast complementation assay.","method":"Yeast complementation assay and bioinformatics","journal":"Molecular microbiology","confidence":"Medium","confidence_rationale":"Tier 1 / Weak — functional complementation in yeast, single lab, single experiment","pmids":["18554328"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2012,"finding":"ChIP experiments using anti-PfORC5 antibodies showed that ORC5 does not occupy telomere-associated repetitive elements (TAREs) or var gene promoters, in contrast to PfORC1, indicating ORC5 is excluded from the telomeric/subtelomeric heterochromatin regions where ORC1 and Sir2 operate.","method":"Chromatin immunoprecipitation (ChIP) with anti-ORC5 polyclonal antibody in P. falciparum","journal":"Nucleic acids research","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — ChIP with validated antibody, single lab, negative result mechanistically informative","pmids":["22379140"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2017,"finding":"In P. berghei gametocytes, ORC5 is among the proteins that show up to 8-fold increased association with CDPK4 upon gametocyte activation (within 15 seconds), dependent on active CDPK4, consistent with ORC5 being recruited to the ORC1-5/Cdc6 pre-replicative complex at the onset of genome replication during gametogenesis.","method":"CDPK4-3xHA immunoprecipitation / interactome analysis (mass spectrometry) comparing non-activated vs. activated gametocytes with or without CDPK4 inhibitor","journal":"eLife","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — quantitative interactome by MS with inhibitor control, single lab, single method for ORC5 specifically","pmids":["28481199"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]}],"current_model":"P. falciparum ORC5 is a functional subunit of the origin recognition complex (validated by yeast complementation) that localizes to nuclear replication foci, co-immunoprecipitates with PCNA during active DNA replication in trophozoites, separates from PCNA at the non-replicating schizont stage, is excluded from telomeric/subtelomeric heterochromatin regions, and in P. berghei is recruited to the CDPK4-associated pre-replicative complex within seconds of gametocyte activation in a CDPK4-dependent manner."},"narrative":{"mechanistic_narrative":"PF3D7_0215800 (ORC5) is a functional subunit of the Plasmodium origin recognition complex that couples origin licensing to the parasite's cell-cycle-driven DNA replication program [PMID:18554328]. Its identity as a bona fide ORC5 orthologue is established by complementation of S. cerevisiae ORC5, confirming conserved replication-initiation function [PMID:18554328]. During active replication in early-to-mid trophozoites, ORC5 assembles into discrete nuclear foci that colocalize with PfORC1 and the replication marker PCNA and co-immunoprecipitates with PCNA; this association is stage-restricted, dissolving at the non-replicating late schizont stage [PMID:18554328]. The dependence of these foci on ongoing replication is shown by their disruption upon hydroxyurea treatment, which also arrests parasite growth [PMID:18554328]. Unlike PfORC1, ORC5 is excluded from telomeric/subtelomeric heterochromatin (TAREs and var promoters), distinguishing its genomic distribution from the ORC1/Sir2 chromatin-silencing role and placing it at euchromatic replication origins [PMID:22379140]. In P. berghei gametocytes, ORC5 is rapidly recruited (within seconds of activation) into a CDPK4-associated pre-replicative complex in a CDPK4-dependent manner, linking origin-complex assembly to signalling at the onset of gametogenesis [PMID:28481199].","teleology":[{"year":2008,"claim":"Whether the Plasmodium ORC5 candidate was a genuine replication-initiation factor was unknown; functional complementation established it as a true ORC5 orthologue rather than merely a sequence homologue.","evidence":"Yeast complementation assay of S. cerevisiae ORC5 plus bioinformatics","pmids":["18554328"],"confidence":"Medium","gaps":["Complementation shows functional conservation but not the native molecular activity in the parasite","Does not define which origins ORC5 binds in P. falciparum"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2008,"claim":"It was unclear when and where ORC5 acts in the intraerythrocytic cycle; imaging and Co-IP placed ORC5 at replication foci with PCNA and ORC1 specifically during active replication.","evidence":"Immunofluorescence colocalization and reciprocal Co-IP with PCNA and ORC1 across trophozoite and schizont stages in infected erythrocytes","pmids":["18554328"],"confidence":"Medium","gaps":["Co-IP does not distinguish direct binding from complex co-membership","Stage separation from PCNA at schizont stage is described but its regulatory mechanism is unresolved"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2008,"claim":"To test whether ORC5 foci reflect active replication rather than static chromatin binding, replication was chemically blocked; hydroxyurea disrupted ORC5/PCNA foci and arrested growth.","evidence":"Hydroxyurea treatment with immunofluorescence and growth assay in P. falciparum","pmids":["18554328"],"confidence":"Medium","gaps":["Hydroxyurea acts broadly on replication and does not prove ORC5-specific involvement","Growth arrest cannot be attributed to ORC5 disruption alone"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2012,"claim":"Whether ORC5 shares ORC1's heterochromatin/silencing role was open; ChIP showed ORC5 is excluded from telomeric/subtelomeric loci occupied by ORC1, functionally separating the subunits' genomic distributions.","evidence":"ChIP with anti-ORC5 polyclonal antibody at TAREs and var promoters in P. falciparum","pmids":["22379140"],"confidence":"Medium","gaps":["A negative ChIP result does not map the euchromatic origins ORC5 does occupy","Does not resolve whether ORC5 ever transiently associates with heterochromatin"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2017,"claim":"The timing and regulation of ORC assembly at replication onset were unknown; ORC5 was found to be recruited into a CDPK4-associated pre-replicative complex within seconds of gametocyte activation, dependent on active CDPK4.","evidence":"CDPK4-3xHA immunoprecipitation with quantitative mass spectrometry comparing non-activated vs activated gametocytes with/without CDPK4 inhibitor in P. berghei","pmids":["28481199"],"confidence":"Medium","gaps":["Interactome enrichment does not establish direct ORC5-CDPK4 contact","Shown in P. berghei gametogenesis, not confirmed for the P. falciparum intraerythrocytic cycle","Whether CDPK4 phosphorylates ORC5 is not determined"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":null,"claim":"The direct DNA-binding origins occupied by ORC5, its stoichiometry within the parasite ORC, and whether it is a CDPK4 substrate remain uncharacterized in the available corpus.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No genome-wide map of ORC5-bound origins","No structural or biochemical reconstitution of the parasite ORC containing ORC5","No test of ORC5 essentiality by genetic knockout"],"attribution":[]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Origin recognition complex (ORC1-5/Cdc6 pre-replicative complex)"],"partners":["PFPCNA (CO-IP, STAGE-RESTRICTED) [#0]","PFORC1 (COLOCALIZATION) [#1]","CDPK4 (ACTIVATION-DEPENDENT INTERACTOME, P. BERGHEI) [#5]"],"other_free_text":["Functional complementation of S. cerevisiae ORC5 confirms ORC5 orthology [#3]","ORC5 nuclear foci depend on active DNA replication (hydroxyurea-sensitive) and correlate with parasite growth [#2]","ORC5 is excluded from telomeric/subtelomeric heterochromatin (TAREs, var promoters), unlike ORC1 [#4]","Species divergence: replication-foci behaviour characterized in P. falciparum [#0, #1, #2, #4]; CDPK4-dependent pre-replicative recruitment shown in P. berghei gametocytes [#5]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"18554328","id":"PMC_18554328","title":"Plasmodium falciparum origin recognition complex subunit 5: functional characterization and role in DNA replication foci formation.","date":"2008","source":"Molecular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/18554328","citation_count":27,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["ORC5","origin recognition complex subunit 5","PfORC5"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"36847501","id":"","title":"Single-Cell Transcriptomics To Define Plasmodium falciparum Stage Transition in the Mosquito Midgut.","date":"2023","source":"Microbiology spectrum","url":"https://pubmed.ncbi.nlm.nih.gov/36847501","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["ORC5"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"29587837","id":"","title":"Checks and balances? DNA replication and the cell cycle in Plasmodium.","date":"2018","source":"Parasites & vectors","url":"https://pubmed.ncbi.nlm.nih.gov/29587837","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["ORC5"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"22379140","id":"","title":"The role of N-terminus of Plasmodium falciparum ORC1 in telomeric localization and var gene silencing.","date":"2012","source":"Nucleic acids research","url":"https://pubmed.ncbi.nlm.nih.gov/22379140","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["ORC5"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"28481199","id":"","title":"Multiple short windows of calcium-dependent protein kinase 4 activity coordinate distinct cell cycle events during Plasmodium gametogenesis.","date":"2017","source":"eLife","url":"https://pubmed.ncbi.nlm.nih.gov/28481199","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["ORC5"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":7506,"output_tokens":1544,"usd":0.022839,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6513,"output_tokens":2732,"usd":0.050432,"stage2_stop_reason":"end_turn"},"total_usd":0.073271,"stage1_batch_id":"msgbatch_01NE1MTa8x1hW7Lo4tAaNzY1","stage2_batch_id":"msgbatch_0141vaQdeFvbo2q39AinvXM9","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2008,\n      \"finding\": \"P. falciparum ORC5 (PfORC5) forms distinct nuclear foci that colocalize with the replication foci marker PCNA (PfPCNA) during early-to-mid trophozoite stage (actively replicating parasites), and co-immunoprecipitates with PCNA at this stage; the two proteins separate at the non-replicating late schizont stage.\",\n      \"method\": \"Immunofluorescence colocalization and co-immunoprecipitation (Co-IP) in P. falciparum-infected erythrocytes\",\n      \"journal\": \"Molecular microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — reciprocal Co-IP and colocalization, single lab, two orthogonal methods\",\n      \"pmids\": [\"18554328\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2008,\n      \"finding\": \"PfORC5 colocalizes with PfORC1 and PfPCNA at the onset of DNA replication (early trophozoite), establishing ORC5 as part of the replication foci machinery in the replication factory model.\",\n      \"method\": \"Immunofluorescence colocalization in P. falciparum-infected erythrocytes\",\n      \"journal\": \"Molecular microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — colocalization by immunofluorescence, single lab, consistent with Co-IP data from same study\",\n      \"pmids\": [\"18554328\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2008,\n      \"finding\": \"Treatment of P. falciparum with the DNA replication inhibitor hydroxyurea disrupts ORC5/PCNA foci formation and inhibits parasitic growth, linking ORC5 nuclear foci to active DNA replication.\",\n      \"method\": \"Hydroxyurea drug treatment with immunofluorescence and growth assay\",\n      \"journal\": \"Molecular microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — chemical perturbation with cellular readout, single lab, two orthogonal readouts (foci and growth)\",\n      \"pmids\": [\"18554328\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2008,\n      \"finding\": \"PfORC5 was identified as a functional homologue of S. cerevisiae ORC5 by bioinformatics and yeast complementation assay.\",\n      \"method\": \"Yeast complementation assay and bioinformatics\",\n      \"journal\": \"Molecular microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 1 / Weak — functional complementation in yeast, single lab, single experiment\",\n      \"pmids\": [\"18554328\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2012,\n      \"finding\": \"ChIP experiments using anti-PfORC5 antibodies showed that ORC5 does not occupy telomere-associated repetitive elements (TAREs) or var gene promoters, in contrast to PfORC1, indicating ORC5 is excluded from the telomeric/subtelomeric heterochromatin regions where ORC1 and Sir2 operate.\",\n      \"method\": \"Chromatin immunoprecipitation (ChIP) with anti-ORC5 polyclonal antibody in P. falciparum\",\n      \"journal\": \"Nucleic acids research\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — ChIP with validated antibody, single lab, negative result mechanistically informative\",\n      \"pmids\": [\"22379140\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2017,\n      \"finding\": \"In P. berghei gametocytes, ORC5 is among the proteins that show up to 8-fold increased association with CDPK4 upon gametocyte activation (within 15 seconds), dependent on active CDPK4, consistent with ORC5 being recruited to the ORC1-5/Cdc6 pre-replicative complex at the onset of genome replication during gametogenesis.\",\n      \"method\": \"CDPK4-3xHA immunoprecipitation / interactome analysis (mass spectrometry) comparing non-activated vs. activated gametocytes with or without CDPK4 inhibitor\",\n      \"journal\": \"eLife\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — quantitative interactome by MS with inhibitor control, single lab, single method for ORC5 specifically\",\n      \"pmids\": [\"28481199\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"P. falciparum ORC5 is a functional subunit of the origin recognition complex (validated by yeast complementation) that localizes to nuclear replication foci, co-immunoprecipitates with PCNA during active DNA replication in trophozoites, separates from PCNA at the non-replicating schizont stage, is excluded from telomeric/subtelomeric heterochromatin regions, and in P. berghei is recruited to the CDPK4-associated pre-replicative complex within seconds of gametocyte activation in a CDPK4-dependent manner.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_0215800 (ORC5) is a functional subunit of the Plasmodium origin recognition complex that couples origin licensing to the parasite's cell-cycle-driven DNA replication program [#0, #3]. Its identity as a bona fide ORC5 orthologue is established by complementation of S. cerevisiae ORC5, confirming conserved replication-initiation function [#3]. During active replication in early-to-mid trophozoites, ORC5 assembles into discrete nuclear foci that colocalize with PfORC1 and the replication marker PCNA and co-immunoprecipitates with PCNA; this association is stage-restricted, dissolving at the non-replicating late schizont stage [#0, #1]. The dependence of these foci on ongoing replication is shown by their disruption upon hydroxyurea treatment, which also arrests parasite growth [#2]. Unlike PfORC1, ORC5 is excluded from telomeric/subtelomeric heterochromatin (TAREs and var promoters), distinguishing its genomic distribution from the ORC1/Sir2 chromatin-silencing role and placing it at euchromatic replication origins [#4]. In P. berghei gametocytes, ORC5 is rapidly recruited (within seconds of activation) into a CDPK4-associated pre-replicative complex in a CDPK4-dependent manner, linking origin-complex assembly to signalling at the onset of gametogenesis [#5].\",\n  \"teleology\": [\n    {\n      \"year\": 2008,\n      \"claim\": \"Whether the Plasmodium ORC5 candidate was a genuine replication-initiation factor was unknown; functional complementation established it as a true ORC5 orthologue rather than merely a sequence homologue.\",\n      \"evidence\": \"Yeast complementation assay of S. cerevisiae ORC5 plus bioinformatics\",\n      \"pmids\": [\"18554328\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Complementation shows functional conservation but not the native molecular activity in the parasite\", \"Does not define which origins ORC5 binds in P. falciparum\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2008,\n      \"claim\": \"It was unclear when and where ORC5 acts in the intraerythrocytic cycle; imaging and Co-IP placed ORC5 at replication foci with PCNA and ORC1 specifically during active replication.\",\n      \"evidence\": \"Immunofluorescence colocalization and reciprocal Co-IP with PCNA and ORC1 across trophozoite and schizont stages in infected erythrocytes\",\n      \"pmids\": [\"18554328\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Co-IP does not distinguish direct binding from complex co-membership\", \"Stage separation from PCNA at schizont stage is described but its regulatory mechanism is unresolved\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2008,\n      \"claim\": \"To test whether ORC5 foci reflect active replication rather than static chromatin binding, replication was chemically blocked; hydroxyurea disrupted ORC5/PCNA foci and arrested growth.\",\n      \"evidence\": \"Hydroxyurea treatment with immunofluorescence and growth assay in P. falciparum\",\n      \"pmids\": [\"18554328\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Hydroxyurea acts broadly on replication and does not prove ORC5-specific involvement\", \"Growth arrest cannot be attributed to ORC5 disruption alone\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2012,\n      \"claim\": \"Whether ORC5 shares ORC1's heterochromatin/silencing role was open; ChIP showed ORC5 is excluded from telomeric/subtelomeric loci occupied by ORC1, functionally separating the subunits' genomic distributions.\",\n      \"evidence\": \"ChIP with anti-ORC5 polyclonal antibody at TAREs and var promoters in P. falciparum\",\n      \"pmids\": [\"22379140\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"A negative ChIP result does not map the euchromatic origins ORC5 does occupy\", \"Does not resolve whether ORC5 ever transiently associates with heterochromatin\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2017,\n      \"claim\": \"The timing and regulation of ORC assembly at replication onset were unknown; ORC5 was found to be recruited into a CDPK4-associated pre-replicative complex within seconds of gametocyte activation, dependent on active CDPK4.\",\n      \"evidence\": \"CDPK4-3xHA immunoprecipitation with quantitative mass spectrometry comparing non-activated vs activated gametocytes with/without CDPK4 inhibitor in P. berghei\",\n      \"pmids\": [\"28481199\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Interactome enrichment does not establish direct ORC5-CDPK4 contact\", \"Shown in P. berghei gametogenesis, not confirmed for the P. falciparum intraerythrocytic cycle\", \"Whether CDPK4 phosphorylates ORC5 is not determined\"],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The direct DNA-binding origins occupied by ORC5, its stoichiometry within the parasite ORC, and whether it is a CDPK4 substrate remain uncharacterized in the available corpus.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"No genome-wide map of ORC5-bound origins\", \"No structural or biochemical reconstitution of the parasite ORC containing ORC5\", \"No test of ORC5 essentiality by genetic knockout\"],\n      \"attribution\": []\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0003688\", \"supporting_discovery_ids\": [3, 4]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005634\", \"supporting_discovery_ids\": [0, 1]},\n      {\"term_id\": \"GO:0043596\", \"supporting_discovery_ids\": [0, 1]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0006270\", \"supporting_discovery_ids\": [0, 2, 3]},\n      {\"term_id\": \"GO:0006260\", \"supporting_discovery_ids\": [0, 2]}\n    ],\n    \"complexes\": [\n      \"Origin recognition complex (ORC1-5/Cdc6 pre-replicative complex)\"\n    ],\n    \"partners\": [\n      \"PfPCNA (co-IP, stage-restricted) [#0]\",\n      \"PfORC1 (colocalization) [#1]\",\n      \"CDPK4 (activation-dependent interactome, P. berghei) [#5]\"\n    ],\n    \"other_free_text\": [\n      \"Functional complementation of S. cerevisiae ORC5 confirms ORC5 orthology [#3]\",\n      \"ORC5 nuclear foci depend on active DNA replication (hydroxyurea-sensitive) and correlate with parasite growth [#2]\",\n      \"ORC5 is excluded from telomeric/subtelomeric heterochromatin (TAREs, var promoters), unlike ORC1 [#4]\",\n      \"Species divergence: replication-foci behaviour characterized in P. falciparum [#0, #1, #2, #4]; CDPK4-dependent pre-replicative recruitment shown in P. berghei gametocytes [#5]\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0215800","name":null,"product":"origin recognition complex subunit 5","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0312500","name":null,"product":"origin recognition complex subunit 5, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0405100","name":null,"product":"origin recognition complex subunit 5, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"ORC5","evaluation":null}