{"gene":"PF3D7_0214900","run_date":"2026-09-07T10:55:51","timeline":{"discoveries":[{"year":2008,"finding":"RON6 (PF3D7_0214900) localizes to the rhoptry neck of P. falciparum merozoites and is transferred to the newly formed parasitophorous vacuole membrane during red blood cell invasion, as shown by immunolocalization experiments.","method":"Immunofluorescence/electron microscopy localization in P. falciparum merozoites and during invasion","journal":"International journal for parasitology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — direct subcellular localization with functional consequence (transfer to PVM during invasion), single lab, two methods (IFA + EM)","pmids":["19073187"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2008,"finding":"The gene encoding RON6 (PF3D7_0214900) is refractory to disruption by integration that truncates the coding sequence, indicating it is essential for the P. falciparum asexual life-cycle. Additionally, the cysteine-rich domain cannot be truncated without loss of function, indicating this domain is functionally important.","method":"Transfection/attempted gene disruption in P. falciparum; failed integration confirmed by PCR","journal":"International journal for parasitology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — transfection refractoriness assay demonstrating essentiality, single lab, single method but with domain-level functional inference","pmids":["19073187"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2008,"finding":"RON6 (PF3D7_0214900) contains a novel cysteine-rich domain that is conserved across Apicomplexan parasites, placing it structurally within the Plasmodium invasion machinery.","method":"Bioinformatic domain analysis of genome/transcriptome data combined with experimental characterization","journal":"International journal for parasitology","confidence":"Low","confidence_rationale":"Tier 4 / Weak — domain identification based on sequence analysis; experimental validation of domain conservation was not biochemically reconstituted","pmids":["19073187"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2023,"finding":"Conditional ER sequestration of RON6 (PF3D7_0214900) using the knockER (DiCre-mediated KDEL ER-retrieval) system confirmed its essentiality in P. falciparum blood-stage invasion, demonstrating that diverting RON6 to the ER away from its rhoptry destination disrupts parasite function.","method":"DiCre-mediated conditional knockER (inducible KDEL ER-retrieval fusion) loss-of-function in P. falciparum","journal":"Proceedings of the National Academy of Sciences of the United States of America","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — clean conditional loss-of-function with defined cellular phenotype (essentiality confirmed), single lab, novel orthogonal method","pmids":["37552754"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2024,"finding":"RON6 (PF3D7_0214900) physically interacts with the P. falciparum sporozoite/merozoite protein Pf34, suggesting RON6 is a member of a functionally important invasion complex. Conditional genetic deletion of RON6 confirmed its essentiality in the blood stage.","method":"Plate-based ectodomain protein-protein interaction screen between recombinant proteins; conditional genetic deletion in P. falciparum","journal":"Malaria journal","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — protein-protein interaction identified by biochemical screen, confirmed by conditional genetic deletion, single lab but two orthogonal methods","pmids":["38755636"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2025,"finding":"P. berghei RON6 (PbRON6), the putative ortholog of PF3D7_0214900/PfRON6, localizes to the sporozoite membrane with its C-terminal domain extracellular. Genetic deletion (PbΔron6) demonstrated that RON6 is required for RBC invasion by merozoites, hepatocyte invasion by sporozoites, and liver-stage development. Loss of RON6 leads to reduced infectivity and virulence in mice.","method":"GFP/epitope-tag localization, genetic knockout (PbΔron6), mouse infection assays, liver-stage culture","journal":"mBio","confidence":"High","confidence_rationale":"Tier 2 / Strong — clean gene knockout with multiple defined phenotypic readouts (merozoite invasion, sporozoite invasion, liver-stage development, virulence), direct localization with topology determination, multiple orthogonal methods in one study","pmids":["40996030"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]}],"current_model":"RON6 (PF3D7_0214900) is an essential rhoptry neck protein in Plasmodium falciparum that localizes to the rhoptry neck of merozoites, is transferred to the parasitophorous vacuole during red blood cell invasion, contains a functionally critical cysteine-rich domain conserved across Apicomplexa, physically interacts with the sporozoite/merozoite protein Pf34 as part of an invasion complex, and—as demonstrated by its P. berghei ortholog—is required for both merozoite invasion of RBCs and sporozoite invasion of hepatocytes as well as liver-stage development, with its C-terminal domain oriented extracellularly on the sporozoite surface."},"narrative":{"mechanistic_narrative":"RON6 (PF3D7_0214900) is an essential rhoptry neck protein of Plasmodium falciparum that functions in host-cell invasion by the merozoite [PMID:19073187, PMID:37552754]. It localizes to the rhoptry neck of merozoites and is discharged onto the newly formed parasitophorous vacuole membrane during red blood cell invasion [PMID:19073187]. The protein carries a novel cysteine-rich domain conserved across Apicomplexa, and this domain is functionally required, as it cannot be truncated without loss of function [PMID:19073187]. RON6 is refractory to gene disruption, and both conditional ER sequestration (knockER) and conditional genetic deletion independently confirm that it is essential for blood-stage invasion [PMID:19073187, PMID:37552754, PMID:38755636]. RON6 physically associates with the sporozoite/merozoite protein Pf34, placing it within an invasion complex [PMID:38755636]. Its role is broadened by its P. berghei ortholog PbRON6, which localizes to the sporozoite membrane with its C-terminal domain oriented extracellularly and is required for merozoite invasion of RBCs, sporozoite invasion of hepatocytes, and liver-stage development, with loss reducing infectivity and virulence in mice [PMID:40996030]. Beyond these findings, the biochemical mechanism by which RON6 promotes invasion has not been characterized in the available corpus.","teleology":[{"year":2008,"claim":"Establishing where RON6 acts and whether it is essential was the first step in placing it within the invasion machinery.","evidence":"Immunofluorescence and electron microscopy localization plus attempted gene disruption in P. falciparum merozoites","pmids":["19073187"],"confidence":"Medium","gaps":["The molecular function of RON6 at the PVM was not defined","Essentiality was inferred from disruption refractoriness rather than a conditional system"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2008,"claim":"Identifying a conserved cysteine-rich domain established a structural feature required for RON6 function and linked it to the broader Apicomplexan invasion apparatus.","evidence":"Bioinformatic domain analysis combined with truncation showing loss of function in P. falciparum","pmids":["19073187"],"confidence":"Low","gaps":["Domain conservation was not biochemically reconstituted","The molecular activity of the cysteine-rich domain is unknown"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2023,"claim":"A conditional loss-of-function system was needed to move beyond disruption refractoriness and directly demonstrate that RON6 is required for blood-stage invasion.","evidence":"DiCre-mediated conditional ER-retrieval (knockER) sequestration in P. falciparum","pmids":["37552754"],"confidence":"Medium","gaps":["Does not identify RON6 binding partners or its step in invasion","Phenotype reflects mislocalization rather than complete protein depletion"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2024,"claim":"Whether RON6 acts alone or within a complex was addressed by testing direct protein-protein interactions, identifying Pf34 as a partner.","evidence":"Plate-based recombinant ectodomain interaction screen plus conditional genetic deletion in P. falciparum","pmids":["38755636"],"confidence":"Medium","gaps":["The RON6-Pf34 interaction was shown for recombinant ectodomains, not confirmed in native complexes","Functional consequence of the interaction for invasion was not tested"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2025,"claim":"The full breadth of RON6 function across life-cycle stages and its membrane topology were resolved using the P. berghei ortholog, extending its role beyond blood-stage invasion to sporozoite invasion and liver-stage development.","evidence":"GFP/epitope-tag localization, genetic knockout, mouse infection, and liver-stage culture in P. berghei","pmids":["40996030"],"confidence":"High","gaps":["Findings are in P. berghei; the multi-stage role has not been demonstrated directly for PF3D7_0214900","The molecular mechanism by which RON6 enables invasion at each stage remains undefined","Whether the Pf34 interaction operates in the sporozoite/liver stage is unknown"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":null,"claim":"The biochemical mechanism by which RON6 and its cysteine-rich domain promote host-cell invasion, and the composition and function of its native invasion complex, remain unresolved.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No molecular activity assigned to the cysteine-rich domain","No native complex composition or structural model","No mechanistic step in the invasion pathway defined for RON6"],"attribution":[]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["RON6-Pf34 invasion complex (recombinant ectodomain interaction) [#4]"],"partners":["PF34 [#4]"],"other_free_text":["Essential rhoptry neck protein; refractory to gene disruption and confirmed essential for blood-stage invasion by conditional knockER and conditional deletion [#1, #3, #4]","Contains a novel cysteine-rich domain conserved across Apicomplexa that is required for function [#2, #1]","Transferred to the parasitophorous vacuole membrane during RBC invasion [#0]","P. berghei ortholog PbRON6 required for merozoite RBC invasion, sporozoite hepatocyte invasion, and liver-stage development, with C-terminal domain extracellular on the sporozoite [#5]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"19073187","id":"PMC_19073187","title":"Characterisation of PfRON6, a Plasmodium falciparum rhoptry neck protein with a novel cysteine-rich domain.","date":"2008","source":"International journal for parasitology","url":"https://pubmed.ncbi.nlm.nih.gov/19073187","citation_count":19,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PfRON6"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"37552754","id":"PMC_37552754","title":"Knock-sideways by inducible ER retrieval enables a unique approach for studying Plasmodium-secreted proteins.","date":"2023","source":"Proceedings of the National Academy of Sciences of the United States of America","url":"https://pubmed.ncbi.nlm.nih.gov/37552754","citation_count":9,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["RON6"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"38755636","id":"PMC_38755636","title":"A screen for Plasmodium falciparum sporozoite surface protein binding to human hepatocyte surface receptors identifies novel host-pathogen interactions.","date":"2024","source":"Malaria journal","url":"https://pubmed.ncbi.nlm.nih.gov/38755636","citation_count":4,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PfRON6"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"40996030","id":"PMC_40996030","title":"Plasmodium berghei rhoptry neck protein 6 maintains parasite infectivity and virulence.","date":"2025","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/40996030","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["RON6","PfRON6","PbRON6"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":5277,"output_tokens":1688,"usd":0.020576,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6771,"output_tokens":2655,"usd":0.050115,"stage2_stop_reason":"end_turn"},"total_usd":0.070691,"stage1_batch_id":"msgbatch_01DbiymDmubkPpYvdbAu5a7V","stage2_batch_id":"msgbatch_012TbrWvMRH129T7PQ4SmvrJ","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2008,\n      \"finding\": \"RON6 (PF3D7_0214900) localizes to the rhoptry neck of P. falciparum merozoites and is transferred to the newly formed parasitophorous vacuole membrane during red blood cell invasion, as shown by immunolocalization experiments.\",\n      \"method\": \"Immunofluorescence/electron microscopy localization in P. falciparum merozoites and during invasion\",\n      \"journal\": \"International journal for parasitology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — direct subcellular localization with functional consequence (transfer to PVM during invasion), single lab, two methods (IFA + EM)\",\n      \"pmids\": [\"19073187\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2008,\n      \"finding\": \"The gene encoding RON6 (PF3D7_0214900) is refractory to disruption by integration that truncates the coding sequence, indicating it is essential for the P. falciparum asexual life-cycle. Additionally, the cysteine-rich domain cannot be truncated without loss of function, indicating this domain is functionally important.\",\n      \"method\": \"Transfection/attempted gene disruption in P. falciparum; failed integration confirmed by PCR\",\n      \"journal\": \"International journal for parasitology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — transfection refractoriness assay demonstrating essentiality, single lab, single method but with domain-level functional inference\",\n      \"pmids\": [\"19073187\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2008,\n      \"finding\": \"RON6 (PF3D7_0214900) contains a novel cysteine-rich domain that is conserved across Apicomplexan parasites, placing it structurally within the Plasmodium invasion machinery.\",\n      \"method\": \"Bioinformatic domain analysis of genome/transcriptome data combined with experimental characterization\",\n      \"journal\": \"International journal for parasitology\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 4 / Weak — domain identification based on sequence analysis; experimental validation of domain conservation was not biochemically reconstituted\",\n      \"pmids\": [\"19073187\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"Conditional ER sequestration of RON6 (PF3D7_0214900) using the knockER (DiCre-mediated KDEL ER-retrieval) system confirmed its essentiality in P. falciparum blood-stage invasion, demonstrating that diverting RON6 to the ER away from its rhoptry destination disrupts parasite function.\",\n      \"method\": \"DiCre-mediated conditional knockER (inducible KDEL ER-retrieval fusion) loss-of-function in P. falciparum\",\n      \"journal\": \"Proceedings of the National Academy of Sciences of the United States of America\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — clean conditional loss-of-function with defined cellular phenotype (essentiality confirmed), single lab, novel orthogonal method\",\n      \"pmids\": [\"37552754\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2024,\n      \"finding\": \"RON6 (PF3D7_0214900) physically interacts with the P. falciparum sporozoite/merozoite protein Pf34, suggesting RON6 is a member of a functionally important invasion complex. Conditional genetic deletion of RON6 confirmed its essentiality in the blood stage.\",\n      \"method\": \"Plate-based ectodomain protein-protein interaction screen between recombinant proteins; conditional genetic deletion in P. falciparum\",\n      \"journal\": \"Malaria journal\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — protein-protein interaction identified by biochemical screen, confirmed by conditional genetic deletion, single lab but two orthogonal methods\",\n      \"pmids\": [\"38755636\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"P. berghei RON6 (PbRON6), the putative ortholog of PF3D7_0214900/PfRON6, localizes to the sporozoite membrane with its C-terminal domain extracellular. Genetic deletion (PbΔron6) demonstrated that RON6 is required for RBC invasion by merozoites, hepatocyte invasion by sporozoites, and liver-stage development. Loss of RON6 leads to reduced infectivity and virulence in mice.\",\n      \"method\": \"GFP/epitope-tag localization, genetic knockout (PbΔron6), mouse infection assays, liver-stage culture\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Strong — clean gene knockout with multiple defined phenotypic readouts (merozoite invasion, sporozoite invasion, liver-stage development, virulence), direct localization with topology determination, multiple orthogonal methods in one study\",\n      \"pmids\": [\"40996030\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"RON6 (PF3D7_0214900) is an essential rhoptry neck protein in Plasmodium falciparum that localizes to the rhoptry neck of merozoites, is transferred to the parasitophorous vacuole during red blood cell invasion, contains a functionally critical cysteine-rich domain conserved across Apicomplexa, physically interacts with the sporozoite/merozoite protein Pf34 as part of an invasion complex, and—as demonstrated by its P. berghei ortholog—is required for both merozoite invasion of RBCs and sporozoite invasion of hepatocytes as well as liver-stage development, with its C-terminal domain oriented extracellularly on the sporozoite surface.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"RON6 (PF3D7_0214900) is an essential rhoptry neck protein of Plasmodium falciparum that functions in host-cell invasion by the merozoite [#0, #3]. It localizes to the rhoptry neck of merozoites and is discharged onto the newly formed parasitophorous vacuole membrane during red blood cell invasion [#0]. The protein carries a novel cysteine-rich domain conserved across Apicomplexa, and this domain is functionally required, as it cannot be truncated without loss of function [#1, #2]. RON6 is refractory to gene disruption, and both conditional ER sequestration (knockER) and conditional genetic deletion independently confirm that it is essential for blood-stage invasion [#1, #3, #4]. RON6 physically associates with the sporozoite/merozoite protein Pf34, placing it within an invasion complex [#4]. Its role is broadened by its P. berghei ortholog PbRON6, which localizes to the sporozoite membrane with its C-terminal domain oriented extracellularly and is required for merozoite invasion of RBCs, sporozoite invasion of hepatocytes, and liver-stage development, with loss reducing infectivity and virulence in mice [#5]. Beyond these findings, the biochemical mechanism by which RON6 promotes invasion has not been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2008,\n      \"claim\": \"Establishing where RON6 acts and whether it is essential was the first step in placing it within the invasion machinery.\",\n      \"evidence\": \"Immunofluorescence and electron microscopy localization plus attempted gene disruption in P. falciparum merozoites\",\n      \"pmids\": [\n        \"19073187\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"The molecular function of RON6 at the PVM was not defined\",\n        \"Essentiality was inferred from disruption refractoriness rather than a conditional system\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": 2008,\n      \"claim\": \"Identifying a conserved cysteine-rich domain established a structural feature required for RON6 function and linked it to the broader Apicomplexan invasion apparatus.\",\n      \"evidence\": \"Bioinformatic domain analysis combined with truncation showing loss of function in P. falciparum\",\n      \"pmids\": [\n        \"19073187\"\n      ],\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"\",\n      \"gaps\": [\n        \"Domain conservation was not biochemically reconstituted\",\n        \"The molecular activity of the cysteine-rich domain is unknown\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"A conditional loss-of-function system was needed to move beyond disruption refractoriness and directly demonstrate that RON6 is required for blood-stage invasion.\",\n      \"evidence\": \"DiCre-mediated conditional ER-retrieval (knockER) sequestration in P. falciparum\",\n      \"pmids\": [\n        \"37552754\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Does not identify RON6 binding partners or its step in invasion\",\n        \"Phenotype reflects mislocalization rather than complete protein depletion\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": 2024,\n      \"claim\": \"Whether RON6 acts alone or within a complex was addressed by testing direct protein-protein interactions, identifying Pf34 as a partner.\",\n      \"evidence\": \"Plate-based recombinant ectodomain interaction screen plus conditional genetic deletion in P. falciparum\",\n      \"pmids\": [\n        \"38755636\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"The RON6-Pf34 interaction was shown for recombinant ectodomains, not confirmed in native complexes\",\n        \"Functional consequence of the interaction for invasion was not tested\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"The full breadth of RON6 function across life-cycle stages and its membrane topology were resolved using the P. berghei ortholog, extending its role beyond blood-stage invasion to sporozoite invasion and liver-stage development.\",\n      \"evidence\": \"GFP/epitope-tag localization, genetic knockout, mouse infection, and liver-stage culture in P. berghei\",\n      \"pmids\": [\n        \"40996030\"\n      ],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Findings are in P. berghei; the multi-stage role has not been demonstrated directly for PF3D7_0214900\",\n        \"The molecular mechanism by which RON6 enables invasion at each stage remains undefined\",\n        \"Whether the Pf34 interaction operates in the sporozoite/liver stage is unknown\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium berghei\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The biochemical mechanism by which RON6 and its cysteine-rich domain promote host-cell invasion, and the composition and function of its native invasion complex, remain unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No molecular activity assigned to the cysteine-rich domain\",\n        \"No native complex composition or structural model\",\n        \"No mechanistic step in the invasion pathway defined for RON6\"\n      ],\n      \"attribution\": []\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\n        \"term_id\": \"GO:0005515\",\n        \"supporting_discovery_ids\": [\n          4\n        ]\n      }\n    ],\n    \"localization\": [\n      {\n        \"term_id\": \"GO:0020008\",\n        \"supporting_discovery_ids\": [\n          0\n        ]\n      },\n      {\n        \"term_id\": \"GO:0005886\",\n        \"supporting_discovery_ids\": [\n          5\n        ]\n      },\n      {\n        \"term_id\": \"GO:0005773\",\n        \"supporting_discovery_ids\": [\n          3\n        ]\n      }\n    ],\n    \"pathway\": [\n      {\n        \"term_id\": \"GO:0044409\",\n        \"supporting_discovery_ids\": [\n          0,\n          5\n        ]\n      },\n      {\n        \"term_id\": \"GO:0006508\",\n        \"supporting_discovery_ids\": []\n      }\n    ],\n    \"complexes\": [\n      \"RON6-Pf34 invasion complex (recombinant ectodomain interaction) [#4]\"\n    ],\n    \"partners\": [\n      \"Pf34 [#4]\"\n    ],\n    \"other_free_text\": [\n      \"Essential rhoptry neck protein; refractory to gene disruption and confirmed essential for blood-stage invasion by conditional knockER and conditional deletion [#1, #3, #4]\",\n      \"Contains a novel cysteine-rich domain conserved across Apicomplexa that is required for function [#2, #1]\",\n      \"Transferred to the parasitophorous vacuole membrane during RBC invasion [#0]\",\n      \"P. berghei ortholog PbRON6 required for merozoite RBC invasion, sporozoite hepatocyte invasion, and liver-stage development, with C-terminal domain extracellular on the sporozoite [#5]\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0214900","name":null,"product":"rhoptry neck protein 6","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0311700","name":null,"product":"rhoptry neck protein 6, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0405900","name":null,"product":"rhoptry neck protein 6, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"RON6","evaluation":null}