{"gene":"PF3D7_0210100","run_date":"2026-09-07T10:55:51","timeline":{"discoveries":[{"year":2014,"finding":"Cryo-EM structure of the P. falciparum 80S ribosome at 3.2 Å resolution places RPL37A (PF3D7_0210100) as a component of the 60S large subunit within the assembled 80S ribosome complex.","method":"Cryo-EM structure determination at 3.2 Å resolution of the intact P. falciparum 80S ribosome","journal":"eLife","confidence":"Medium","confidence_rationale":"Tier 1 / Weak — high-resolution cryo-EM structure of the whole ribosome establishes subunit membership, but no experiment specifically probes RPL37A function or interactions individually; single study","pmids":["24913268"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2015,"finding":"Cryo-EM reconstructions of the P. falciparum 80S ribosome purified from blood-stage schizonts confirm RPL37A (PF3D7_0210100) as a structural component of the 60S subunit in the assembled translation-competent ribosome, and atomic models built by flexible fitting place it within the large subunit architecture.","method":"Cryo-EM with sub-nanometer resolution reconstructions and flexible-fitting atomic model building from schizont-purified ribosomes","journal":"Nucleic acids research","confidence":"Medium","confidence_rationale":"Tier 1 / Weak — cryo-EM structure confirms large-subunit membership but does not experimentally interrogate RPL37A specifically; single study","pmids":["26432834"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"PF3D7_0210100 (RPL37A) is established as a structural component of the 60S large subunit of the P. falciparum 80S cytoplasmic ribosome, as evidenced by its presence in high-resolution cryo-EM structures of the intact ribosome; no experiment has specifically probed its individual function, binding partners, or enzymatic activity beyond its membership in the ribosomal large subunit."},"narrative":{"mechanistic_narrative":"PF3D7_0210100 (RPL37A) is a structural component of the 60S large subunit of the Plasmodium falciparum 80S cytoplasmic ribosome, placed within the large-subunit architecture by high-resolution cryo-EM structures of the intact translation-competent ribosome [PMID:24913268, PMID:26432834]. Its assignment is based on structural membership in the assembled ribosome purified from blood-stage schizonts rather than on any experiment probing RPL37A individually [PMID:26432834]. Beyond this membership in the ribosomal large subunit, no experiment in the available corpus has probed the individual function, binding partners, or enzymatic activity of PF3D7_0210100.","teleology":[{"year":2014,"claim":"Whether PF3D7_0210100 is part of the assembled P. falciparum ribosome was resolved by placing it structurally within the machine.","evidence":"Cryo-EM structure of the intact P. falciparum 80S ribosome at 3.2 Å resolution assigning RPL37A to the 60S large subunit","pmids":["24913268"],"confidence":"Medium","gaps":["No experiment probes RPL37A function, interactions, or essentiality individually","Role in ribosome assembly or translation activity not tested","Position within the subunit inferred from whole-ribosome structure, not isolated study"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2015,"claim":"Whether RPL37A is a stable component of the native, translation-competent ribosome was confirmed using ribosomes isolated from parasites rather than reconstituted material.","evidence":"Cryo-EM reconstructions with flexible-fitting atomic model building from schizont-purified P. falciparum 80S ribosomes place RPL37A within the 60S subunit","pmids":["26432834"],"confidence":"Medium","gaps":["Does not interrogate RPL37A-specific contacts or contributions to subunit function","No functional perturbation (knockdown/knockout) performed","Any parasite-specific role distinct from the general structural role remains untested"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"The individual function, binding partners, essentiality, and any parasite-specific role of PF3D7_0210100 beyond structural membership in the 60S subunit remain uncharacterized.","evidence":"No discovery in the corpus experimentally isolates or perturbs RPL37A","pmids":[],"confidence":"Medium","gaps":["No functional or genetic study of RPL37A","No defined interaction partners beyond co-membership in the large subunit","No structural or mechanistic feature distinguishing it from a generic ribosomal protein"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["60S large ribosomal subunit","80S cytoplasmic ribosome"],"partners":[],"other_free_text":["Structural membership in the P. falciparum 60S large ribosomal subunit is established by cryo-EM of the intact 80S ribosome [#0, #1]; no experiment in the corpus has probed PF3D7_0210100 individually beyond this complex membership."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"24913268","id":"PMC_24913268","title":"Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine.","date":"2014","source":"eLife","url":"https://pubmed.ncbi.nlm.nih.gov/24913268","citation_count":276,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"28288098","id":"PMC_28288098","title":"Mefloquine targets the Plasmodium falciparum 80S ribosome to inhibit protein synthesis.","date":"2017","source":"Nature microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/28288098","citation_count":138,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"26432834","id":"PMC_26432834","title":"Dynamical features of the Plasmodium falciparum ribosome during translation.","date":"2015","source":"Nucleic acids research","url":"https://pubmed.ncbi.nlm.nih.gov/26432834","citation_count":46,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":4084,"output_tokens":635,"usd":0.010889,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5226,"output_tokens":1525,"usd":0.032128,"stage2_stop_reason":"end_turn"},"total_usd":0.043017,"stage1_batch_id":"msgbatch_01DbiymDmubkPpYvdbAu5a7V","stage2_batch_id":"msgbatch_012TbrWvMRH129T7PQ4SmvrJ","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2014,\n      \"finding\": \"Cryo-EM structure of the P. falciparum 80S ribosome at 3.2 Å resolution places RPL37A (PF3D7_0210100) as a component of the 60S large subunit within the assembled 80S ribosome complex.\",\n      \"method\": \"Cryo-EM structure determination at 3.2 Å resolution of the intact P. falciparum 80S ribosome\",\n      \"journal\": \"eLife\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 1 / Weak — high-resolution cryo-EM structure of the whole ribosome establishes subunit membership, but no experiment specifically probes RPL37A function or interactions individually; single study\",\n      \"pmids\": [\"24913268\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2015,\n      \"finding\": \"Cryo-EM reconstructions of the P. falciparum 80S ribosome purified from blood-stage schizonts confirm RPL37A (PF3D7_0210100) as a structural component of the 60S subunit in the assembled translation-competent ribosome, and atomic models built by flexible fitting place it within the large subunit architecture.\",\n      \"method\": \"Cryo-EM with sub-nanometer resolution reconstructions and flexible-fitting atomic model building from schizont-purified ribosomes\",\n      \"journal\": \"Nucleic acids research\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 1 / Weak — cryo-EM structure confirms large-subunit membership but does not experimentally interrogate RPL37A specifically; single study\",\n      \"pmids\": [\"26432834\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"PF3D7_0210100 (RPL37A) is established as a structural component of the 60S large subunit of the P. falciparum 80S cytoplasmic ribosome, as evidenced by its presence in high-resolution cryo-EM structures of the intact ribosome; no experiment has specifically probed its individual function, binding partners, or enzymatic activity beyond its membership in the ribosomal large subunit.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_0210100 (RPL37A) is a structural component of the 60S large subunit of the Plasmodium falciparum 80S cytoplasmic ribosome, placed within the large-subunit architecture by high-resolution cryo-EM structures of the intact translation-competent ribosome [#0, #1]. Its assignment is based on structural membership in the assembled ribosome purified from blood-stage schizonts rather than on any experiment probing RPL37A individually [#1]. Beyond this membership in the ribosomal large subunit, no experiment in the available corpus has probed the individual function, binding partners, or enzymatic activity of PF3D7_0210100.\",\n  \"teleology\": [\n    {\n      \"year\": 2014,\n      \"claim\": \"Whether PF3D7_0210100 is part of the assembled P. falciparum ribosome was resolved by placing it structurally within the machine.\",\n      \"evidence\": \"Cryo-EM structure of the intact P. falciparum 80S ribosome at 3.2 Å resolution assigning RPL37A to the 60S large subunit\",\n      \"pmids\": [\n        \"24913268\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No experiment probes RPL37A function, interactions, or essentiality individually\",\n        \"Role in ribosome assembly or translation activity not tested\",\n        \"Position within the subunit inferred from whole-ribosome structure, not isolated study\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": 2015,\n      \"claim\": \"Whether RPL37A is a stable component of the native, translation-competent ribosome was confirmed using ribosomes isolated from parasites rather than reconstituted material.\",\n      \"evidence\": \"Cryo-EM reconstructions with flexible-fitting atomic model building from schizont-purified P. falciparum 80S ribosomes place RPL37A within the 60S subunit\",\n      \"pmids\": [\n        \"26432834\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Does not interrogate RPL37A-specific contacts or contributions to subunit function\",\n        \"No functional perturbation (knockdown/knockout) performed\",\n        \"Any parasite-specific role distinct from the general structural role remains untested\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The individual function, binding partners, essentiality, and any parasite-specific role of PF3D7_0210100 beyond structural membership in the 60S subunit remain uncharacterized.\",\n      \"evidence\": \"No discovery in the corpus experimentally isolates or perturbs RPL37A\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No functional or genetic study of RPL37A\",\n        \"No defined interaction partners beyond co-membership in the large subunit\",\n        \"No structural or mechanistic feature distinguishing it from a generic ribosomal protein\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\n        \"term_id\": \"GO:0003735\",\n        \"supporting_discovery_ids\": [\n          0,\n          1\n        ]\n      }\n    ],\n    \"localization\": [\n      {\n        \"term_id\": \"GO:0022625\",\n        \"supporting_discovery_ids\": [\n          0,\n          1\n        ]\n      },\n      {\n        \"term_id\": \"GO:0005737\",\n        \"supporting_discovery_ids\": [\n          0,\n          1\n        ]\n      }\n    ],\n    \"pathway\": [\n      {\n        \"term_id\": \"GO:0006412\",\n        \"supporting_discovery_ids\": [\n          0,\n          1\n        ]\n      }\n    ],\n    \"complexes\": [\n      \"60S large ribosomal subunit\",\n      \"80S cytoplasmic ribosome\"\n    ],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Structural membership in the P. falciparum 60S large ribosomal subunit is established by cryo-EM of the intact 80S ribosome [#0, #1]; no experiment in the corpus has probed PF3D7_0210100 individually beyond this complex membership.\"\n    ]\n  }\n}","audit_flag":{"gene":"PF3D7_0210100","tier":"IDENTITY_CHECK","verdict":"identity unverified","subtype":"names_other_cards","uniprot_band":"","rules_fired":"R12","issue":"No cited paper names this card; 1 of 2 cited papers name other catalogue genes (PF3D7_0826700). The summary may describe one of those genes."},"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0210100","name":null,"product":"60S ribosomal protein L37ae, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0307000","name":null,"product":"60S ribosomal protein L37ae, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0411100","name":null,"product":"60S ribosomal protein L37ae, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"RPL37A","evaluation":null}