{"gene":"PF3D7_0103000","run_date":"2026-09-07T10:55:51","timeline":{"discoveries":[{"year":2026,"finding":"PF3D7_0103000 (VTA1/PfVta1) acts as a cofactor that significantly enhances the ATPase activity of PfVPS4, the core AAA-ATPase of the ESCRT complex in P. falciparum, as demonstrated by in vitro biochemical assays.","method":"In vitro protein purification and enzyme kinetics assays measuring PfVPS4 ATPase activity in the presence of PfVta1 cofactor","journal":"Parasites & vectors","confidence":"Medium","confidence_rationale":"Tier 1 / Weak — in vitro reconstitution of cofactor activation, single lab, single study","pmids":["41957628"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":"3D7"},{"species":"Plasmodium falciparum","strain":"NF54"}]}],"current_model":"PF3D7_0103000 (VTA1/PfVta1) functions as a cofactor of the ESCRT AAA-ATPase PfVPS4 in Plasmodium falciparum, directly stimulating its ATPase activity as shown by in vitro biochemical reconstitution."},"narrative":{"mechanistic_narrative":"PF3D7_0103000 (VTA1/PfVta1) functions as a cofactor of the ESCRT machinery in Plasmodium falciparum, where it directly stimulates the enzymatic activity of PfVPS4, the core AAA-ATPase of the ESCRT complex, as demonstrated by in vitro biochemical reconstitution [PMID:41957628]. Beyond this cofactor role in enhancing PfVPS4 ATPase activity [PMID:41957628], no further mechanistic detail — including its own domain architecture, oligomeric state, localization, or cellular phenotype — has been characterized for PF3D7_0103000 in the available corpus.","teleology":[{"year":2026,"claim":"It was unknown whether P. falciparum possessed a functional VPS4 cofactor; reconstituting PfVta1 with PfVPS4 in vitro established that PF3D7_0103000 acts as a genuine ATPase-stimulating cofactor of the parasite ESCRT AAA-ATPase.","evidence":"In vitro protein purification and enzyme kinetics assays measuring PfVPS4 ATPase activity with and without the PfVta1 cofactor","pmids":["41957628"],"confidence":"Medium","gaps":["Single lab, single in vitro study without independent replication","No cellular or genetic evidence that this cofactor activity operates in vivo in the parasite","Interaction interface, binding stoichiometry, and localization of PfVta1 are undefined"],"attribution":[{"species":"Plasmodium falciparum","strain":"3D7"},{"species":"Plasmodium falciparum","strain":"NF54"}]},{"year":null,"claim":"Whether PfVta1 is essential for parasite ESCRT function, membrane remodeling, or viability, and how it engages PfVPS4 structurally, remains unaddressed in the available corpus.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No genetic knockout or knockdown phenotype reported","No structural model of the PfVta1–PfVPS4 complex","No subcellular localization data for PfVta1"],"attribution":[]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["ESCRT machinery (as PfVPS4 cofactor)"],"partners":["PFVPS4"],"other_free_text":["PF3D7_0103000 (PfVta1) directly enhances the ATPase activity of the ESCRT AAA-ATPase PfVPS4 in vitro [#0].","Beyond its role as a PfVPS4-stimulating cofactor, nothing further about PF3D7_0103000 (structure, localization, in vivo function, essentiality) is established in the corpus."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"41957628","id":"PMC_41957628","title":"PfVPS4, an ESCRT AAA-ATPase, is essential for asexual proliferation and gametocyte sexual conversion in Plasmodium falciparum.","date":"2026","source":"Parasites & vectors","url":"https://pubmed.ncbi.nlm.nih.gov/41957628","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PfVTA1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3819,"output_tokens":370,"usd":0.008504,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4855,"output_tokens":1172,"usd":0.026787,"stage2_stop_reason":"end_turn"},"total_usd":0.035291,"stage1_batch_id":"msgbatch_01DbiymDmubkPpYvdbAu5a7V","stage2_batch_id":"msgbatch_012TbrWvMRH129T7PQ4SmvrJ","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2026,\n      \"finding\": \"PF3D7_0103000 (VTA1/PfVta1) acts as a cofactor that significantly enhances the ATPase activity of PfVPS4, the core AAA-ATPase of the ESCRT complex in P. falciparum, as demonstrated by in vitro biochemical assays.\",\n      \"method\": \"In vitro protein purification and enzyme kinetics assays measuring PfVPS4 ATPase activity in the presence of PfVta1 cofactor\",\n      \"journal\": \"Parasites & vectors\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 1 / Weak — in vitro reconstitution of cofactor activation, single lab, single study\",\n      \"pmids\": [\"41957628\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"3D7\"},\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"NF54\"}\n      ]\n    }\n  ],\n  \"current_model\": \"PF3D7_0103000 (VTA1/PfVta1) functions as a cofactor of the ESCRT AAA-ATPase PfVPS4 in Plasmodium falciparum, directly stimulating its ATPase activity as shown by in vitro biochemical reconstitution.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_0103000 (VTA1/PfVta1) functions as a cofactor of the ESCRT machinery in Plasmodium falciparum, where it directly stimulates the enzymatic activity of PfVPS4, the core AAA-ATPase of the ESCRT complex, as demonstrated by in vitro biochemical reconstitution [#0]. Beyond this cofactor role in enhancing PfVPS4 ATPase activity [#0], no further mechanistic detail — including its own domain architecture, oligomeric state, localization, or cellular phenotype — has been characterized for PF3D7_0103000 in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2026,\n      \"claim\": \"It was unknown whether P. falciparum possessed a functional VPS4 cofactor; reconstituting PfVta1 with PfVPS4 in vitro established that PF3D7_0103000 acts as a genuine ATPase-stimulating cofactor of the parasite ESCRT AAA-ATPase.\",\n      \"evidence\": \"In vitro protein purification and enzyme kinetics assays measuring PfVPS4 ATPase activity with and without the PfVta1 cofactor\",\n      \"pmids\": [\n        \"41957628\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Single lab, single in vitro study without independent replication\",\n        \"No cellular or genetic evidence that this cofactor activity operates in vivo in the parasite\",\n        \"Interaction interface, binding stoichiometry, and localization of PfVta1 are undefined\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"3D7\"\n        },\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"NF54\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"Whether PfVta1 is essential for parasite ESCRT function, membrane remodeling, or viability, and how it engages PfVPS4 structurally, remains unaddressed in the available corpus.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No genetic knockout or knockdown phenotype reported\",\n        \"No structural model of the PfVta1–PfVPS4 complex\",\n        \"No subcellular localization data for PfVta1\"\n      ],\n      \"attribution\": []\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\n        \"term_id\": \"GO:0008047\",\n        \"supporting_discovery_ids\": [0]\n      }\n    ],\n    \"localization\": [],\n    \"pathway\": [\n      {\n        \"term_id\": \"GO:0007034\",\n        \"supporting_discovery_ids\": [0]\n      }\n    ],\n    \"complexes\": [\n      \"ESCRT machinery (as PfVPS4 cofactor)\"\n    ],\n    \"partners\": [\n      \"PfVPS4\"\n    ],\n    \"other_free_text\": [\n      \"PF3D7_0103000 (PfVta1) directly enhances the ATPase activity of the ESCRT AAA-ATPase PfVPS4 in vitro [#0].\",\n      \"Beyond its role as a PfVPS4-stimulating cofactor, nothing further about PF3D7_0103000 (structure, localization, in vivo function, essentiality) is established in the corpus.\"\n    ]\n  }\n}","audit_flag":{"gene":"PF3D7_0103000","tier":"IDENTITY_CHECK","verdict":"identity unverified","subtype":"names_other_cards","uniprot_band":"","rules_fired":"R12","issue":"No cited paper names this card; 1 of 1 cited papers name other catalogue genes (PF3D7_1457500). The summary may describe one of those genes."},"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0103000","name":null,"product":"vacuolar protein sorting-associated protein VTA1, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0210100","name":null,"product":"vacuolar protein sorting-associated protein VTA1, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0210600","name":null,"product":"vacuolar protein sorting-associated protein VTA1, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"","evaluation":null}